inclusion body造句

• After the protein refolding of denaturant inclusion body following dialysis , we got the pure recombinant gst - eo protein by gst affinity columns . using the purified protein as coating antigen , an indirect elisa were developed for detecting the anti - eo antibody in the csfv serum by exploring the concentration of coating an tigen and dilution degree of serum
  使用分步透析法对变性的包涵体进行复性，将复性蛋白过gst亲和层析柱得到纯化的gst - eo融合蛋白。以gst - eo融合蛋白为诊断抗原，初步建立了用间接elisa检测猪瘟血清eo抗体的方法。
• Conclusion : it ' s possible to improve the expression efficiency by adjusting the g + c content of tir region and codon usage . replacement the cystein 78 and cystein 96 into serines , higher bioactivity gained compared with control hbfgf protein , and decreased amout of dimer and inclusion body , this suggested that free thiol group of cys78 and cys96 is the main reason of dimer and multimer formation
  结论：通过调整tir区g c含量及增加大肠杆菌偏爱的密码子，确实能提高hbfgf在大肠杆菌中的表达量，而将78和96位上的半眯氨酸替换成丝氨酸后，得到的hbfgf的蛋白活性高于未改构蛋白，二聚体和包涵体减少。
• In this study , the recombinant plasmid pmd - 18t - pea - h3 was cleavaged with ncoi , xhoi and inserted into the expression vector pet - 28c and subsequently subjected to restriction endonuclease analysis and sequencing , the result indicated that the prokaryotic expression vector pet - 28c - pea - h3 was constructed successfully . after the expression plasmid was extracted and transformed into expression hosts bl21 ( de3 ) of e . coli , the transformed hosts were induced by iptg , bysds - page and elisa analysis of host protein . the expression of the objective gene was detected , and it could account for 16 . 28 % of the total host protein . inclusion body was prepared from the incubating expression hosts induced by iptg
  同时将原核表达载体pet - 28c用nco ， xho双酶切，回收酶切产物，将回收的酶切产物pea ， h3 ，载体进行连接，并转入dh5感受态细胞内，培养12 - 18小时后，挑取阳性菌落，经nco ， xho双酶切分析及pcr检测，筛选到阳性克隆，其质粒测序结果表明成功地构建了毒性基因缺失的pea与人组蛋白h3融合基因的原核表达载体。
• The supernatant fraction and the precipitation fractions were analyzed by western blotfor strain dh5 a / pkkfpga , 5 - 10 % pga precursors formed as inclusion bodies in the cytoplasm while no inclusion bodies formed in the periplasm , this suggested most pga precursors were transported to the periplasm and matured to active pga and indicated that the maturation of pga in strain dh5 / pkkfpga was limited by the translocation step
  Western印迹分析表明对于菌株dh5 pkkfpga ， 5 - 10的原前体青霉g素酰化酶在胞内形成了包涵体，说明其成熟的限速步骤在胞内的运输阶段，而菌株dh5 psmlfpga则无明显包涵体形成，说明菌株dh5 psmlfpga改善了青霉素g酰化酶的合成流，因而其表达能力高于菌株dh5 pkkfpga 。